The Buccaneer Barnacles first appeared in the episode "A Bad Case of the Barnacles!",Jake,Izzy and Cubby discover Bucky has a bad case of barnacles and the only way to get rid of them is to find the Zebra Rose which grows on Crimson Isle the home of Red Jessica. Jake inform Red Jessica about their trouble and ask if she knew were to find the Zebra Rose,she informs the young pirates to search through the Valley of Roses to find Zebra Rose. While on the hunt for the rose Captain Hook manages to swipe the rose from the puny pirates and flee back to Crimson Castle with Jake and his crew in pursuit.When Hook reached Crimson Castle he presented the Zebra Rose to his beloved Red Jessica who was very impress with Hook believing he went through all trouble to help Jake and crew ship cure who she give leaving Hook both puzzled and confuse on the matter. Jake and rush back to the docks were Bucky was anchored with a few whiffs from Zebra Rose sent the barnacles flee back into the Never Sea.The Barnacles make one final appearance at the end of the episode making their new home on the hall of Hook's ship the Jolly Roger.